Reproduction Abstracts

Background: As a consequence of cryopreservation of human ovaries preformed in our laboratory we have access to human small antral follicles.Material: Human granulosa cells (GC) from individual small antral follicles (4–6 mm in diameter) were spilt in two and either cultured or snap-frozen immediately after isolation from the follicular fluid. Microarray analysis was used on both the cultured and the non-cultured sample, to examine their gene expres...

Background: FSH is one of the key hormones of mammalian reproduction. FSH signaling may be affected by single-nucleotide polymorphisms residing in the FSH receptor (FSHR) gene. The most pronounced effects of these polymorphisms are likely to be displayed in the follicle fluid, which acts as a reservoir for granulosa cell secreted hormones induced by FSH.Methods: Genetic polymorphisms of FSHR in position -29 (FSHR -29) and in codon 307 and codon ...